12859_2016_876_MOESM1_ESM.jpg (270.1 kB)
Additional file 1: Figure S1. of IPLaminator: an ImageJ plugin for automated binning and quantification of retinal lamination
figure
posted on 2016-01-16, 05:00 authored by Shuai Li, Michael Woodfin, Seth Long, Peter FuerstIPLaminator interface. A, Angle tool was selected to determinate rotation and the degree was displayed in the Fiji interface under tool selection area. B, Rotation tool was used to rotate image so that the RGC layer is facing left and the retina is vertically aligned. C, User interface of IPLaminator, it simply asks user to define 3 elements. First, the image used to define nuclear layer boundaries. Second S2/S4 Plot Profile is an image of ChAT or equivalent staining that will be used to automatically define layers. Last, all image channels that need to be analyzed are selected.