TY - DATA T1 - Supporting data for "Comparative whole genome DNA methylation profiling of cattle sperm and somatic tissues reveals striking hypomethylated patterns in sperm" AU - Benjamin, Rosen D AU - Congjun, Li AU - Curtis, Van Tassell P AU - Derek, Bickhart M AU - Erin, Connor E AU - George, Liu E AU - Liguo, Yang AU - Ransom, Baldwin L AU - Steven, Schroeder G AU - Yang, Zhou DO - 10.5524/100424 UR - http://gigadb.org/dataset/100424 AB - Compared to somatic cells, sperm cells undergo nearly complete reprogramming of DNA methylation and exchange histones by protamine. Although sperm DNA methylation has been studied in humans and other model species, as the most beneficiary species of artificial insemination, its status in cattle is largely unknown. Using whole-genome bisulfite sequencing (WGBS), we profiled the DNA methylome of cattle sperm through comparison with somatic cells from three bovine tissues (mammary gland, brain, and blood). Large differences between cattle sperm and somatic cells were observed in the methylation patterns of global CpGs, pericentromeric satellites, partially methylated domains (PMDs), hypomethylated regions (HMRs), and common repeats. As expected and like other species, we observed low methylation in the promoter regions and high methylation in the bodies of active genes. We detected selective hypomethylation of megabase domains of centromeric satellite clusters, which may be related to chromosome segregation during meiosis and their rapid transcriptional activation upon fertilization. We found more PMDs in sperm cells than in the somatic cells and identified meiosis-related genes like KIF2B and REPIN1, which are hypomethylated in sperm but hypermethylated in somatic cells. Besides the common HMRs around gene promoters which showed substantial differences between sperm and somatic cells, the sperm-specific HMRs also targeted to distinct spermatogenesis-related genes, including BOLL, MAEL, ASZ1, SYCP3, CTCFL, MND1, SPATA22, PLD6, DDX4, RBBP8, FKBP6, and SYCE1. Although common repeats were heavily methylated in both sperm and somatic cells, some hypomethylated repeats were enriched in gene promoters with large variations among tissues. For example, some young Bov-A2 repeats, which belong to the SINE family, were hypomethylated and could affect the promoter structures by introducing new regulatory elements. Our study provides a comprehensive resource for bovine sperm epigenomic research and enables new discoveries about DNA methylation and its role in male fertility. KW - Epigenomic KW - cattle KW - sperm KW - somatic cells KW - dna methylation KW - hypomethylated region PY - 2018 PB - GigaScience Database LA - en ER -