10.3205/GPRAS000008
Herold, C.
C.
Herold
Pflaum, M.
M.
Pflaum
Utz, P.
P.
Utz
Wilhelmi, M.
M.
Wilhelmi
Vogt, P. M.
P. M.
Vogt
Rennekampff, H. O.
H. O.
Rennekampff
Autologous fat transplantation – animal models and methods for in vitro analysis of viability
German Medical Science GMS Publishing House
2012
JournalArticle
610 Medical sciences; Medicine
2012-07-23
2012
en
urn:nbn:de:0183-gpras0000087
gpras000008
text/html
GMS German Plastic, Reconstructive and Aesthetic Surgery; 2:Doc06; ISSN 2193-7052
Since Peer presented his “cell survival theory” it is assumed that the grafted tissue will be implemented in some way into the host tissue. This lead to a big effort to produce a fat graft that is as much viable as possible. Various studies have been performed to analyse viability of fat grafts prepared with different techniques or protocols. All viability tests in vitro permit a non invasive way to analyse human fat grafts. But the influence of processing due to the viability test protocol on viability of fat grafts cannot be totally excluded. Furthermore no normative values exist and we do not know if the test will really measure the real viability of the fat graft. Therefore an in vitro test can only be rated by the following characteristics: a) degree of tissue modification by the test itself, b) specificity on adipose tissue, c) ability to depict the relative rate of viable cells to the total cells of the graft. After all an ideal in vitro test still has to be invented, as all available tests do have their indications but also their disadvantages. Animal models offer in vivo analysis of fat survival, but results can not be transferred directly to humans. If autologous fat transplantation is performed for volume augmentation purposes the technique of lipofilling with the best volume survival rate and the least complications like oil cysts or calcifications would be superior.
GMS German Plastic, Reconstructive and Aesthetic Surgery; 2:Doc06; ISSN 2193-7052