10.4123/SIDR.000002Y.RP
Cauchy, Pierre
Pierre
Cauchy
Benoukraf, Touati
Touati
Benoukraf
Fenouil, Romain
Romain
Fenouil
Koch, Frédéric
Frédéric
Koch
Andrau, Jean-Christophe
Jean-Christophe
Andrau
Ferrier, Pierre
Pierre
Ferrier
Suz12 ChIP-on-chip of mouse embryonic stem cell
Centre d'Immunologie de Marseille-Luminy (CIML), Université de la Méditerranée, INSERM U631, CNRS UMR6102, Parc Scientifique et Technologique de Luminy, Case 906, F13288 Marseille cedex 09, France
2010
Experiment report
Epigenetics and gene regulation
Transcriptomics
Mus musculus
Chromatin
genomic DNA
Transcription factor binding site identification
DNA microarray
INIST-CNRS
Zasadzinski, Alain
Alain
Zasadzinski
2010-07-16
2010
en
10.4123/sidr.000002z.rp
10.1093/bioinformatics/btp075
10.1038/nature04733
209 ko
3 pages
PDF
We aim to identify target sequences of suz12, a member of the polycomb group proteins (PcG) PRC2 (polycomb repressive complex 2). We study the role played by this polycomb complex on genetic plasticity during embryonic development in undifferentiated mouse embryonic stem cells (day 8). Suz12 is required for trimethylation of the tail of histone H3 on lysine 27 (H3K27me3). This epigenetic modification of chromatin induces dynamic repression of many target genes involved in developmental pathways. The experiment reported here aimed to set up the ChIP-on-chip procedure in our lab, and this study was already published by the Young RA group (Boyer LA and al., Nature (2006), 441, 349-353).
Cites: Boyer LA; Plath K; Zeitlinger J; Brambrink T; Medeiros LA; Lee TI; Levine SS; Wernig M;Tajonar A; Ray MK; Bell GW; Otte AP; Vidal M; Gifford DK; Young RA; Jaenisch R. Polycomb complexes repress developmental regulators in murine embryonic. Nature. 2006 May 18;441(7091):349-53. PMID : 16625203
Cites: Benoukraf T; Cauchy P; Fenouil R; Jeanniard A; Koch F; Jaeger S; Thieffry D; Imbert J; Andrau JC; Spicuglia S; Ferrier P. CoCAS: a ChIP-on-chip analysis suite. Bioinformatics. 2009 Apr 1;25(7):954-5. PMID: 19193731