10.5681/APB.2013.043
Ghahramanian Golzar, Mina
Mina
Ghahramanian Golzar
Babri, Shirin
Shirin
Babri
Ataie, Zohre
Zohre
Ataie
Ebrahimi, Hadi
Hadi
Ebrahimi
Mirzaie, Fariba
Fariba
Mirzaie
Mohaddes, Gisou
Gisou
Mohaddes
NPY Receptors Blockade Prevents Anticonvulsant Action of Ghrelin in the Hippocampus of Rat
Tabriz University of Medical Sciences
2013
JournalArticle
610 Medical sciences; Medicine
2013-08-20
2013
en
text/html
Advanced Pharmaceutical Bulletin; eISSN 2251-7308
Purpose: Ghrelin has been shown to have antiepileptic function. However, the underlying mechanisms by which, ghrelin exerts its antiepileptic effects are still unclear. In the present study, we investigated whether neuropeptide Y (NPY) mediates ghrelin anticonvulsant effect in the brain through its Y1, Y2 or Y5 receptors. Methods: Male Wistar rats were bilaterally microinjected with ghrelin 0.3 nmol/μl/side and NPY antagonists; GR231118 (Y1 receptor antagonist), BIIE0246 (Y2 receptor antagonist), CGP71683 (Y5 receptor antagonist) or solvents (Saline, DMSO) into the dorsal hippocampus 20 minutes before ghrelin administration. Thirty minutes after ghrelin microinjection, a single convulsive dose of pentylenetetrazole (PTZ) (50 mg/kg) was injected intraperitoneally (ip). Afterwards, duration of seizure and total seizure score (TSS) were assessed for 30 minutes in all animals. Results: Intrahippocampal injection of 0.3 nmol/μl/side ghrelin decreased duration of seizure and TSS induced by PTZ. The suppression of both duration (p< 0.001) and TSS (plt; 0.001) induced by ghrelin in hippocampus were significantly blocked by GR231118 (10 μg/μl/side), BIIE0246 (400 pmol/μl/side) and CGP 71683A (5 nmol/μl/side). Conclusion: Our findings suggest that NPY Y1, Y2 and Y5 receptors in the hippocampus may somehow mediate the anticonvulsive action of ghrelin. Therefore, it is possible to speculate that ghrelin acts in the hippocampus to modulate seizures via NPY.
Advanced Pharmaceutical Bulletin; eISSN 2251-7308