10.5681/BI.2012.007
Pourhassan-Moghaddam, Mohammad
Mohammad
Pourhassan-Moghaddam
Farhadi, Behrouz
Behrouz
Farhadi
Nejati-Koshki, Kazem
Kazem
Nejati-Koshki
Moharrami, Tamouchin
Tamouchin
Moharrami
Promoters of Cancer Genes for Recombinant Protein Expression in Human Cancer Cell Lines
Tabriz University of Medical Sciences
2012
JournalArticle
610 Medical sciences; Medicine
2012-03-18
2012
en
text/html
BioImpacts; ISSN 2228-5660
Introduction: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Therefore, it is necessary to use new expression systems to overcome the indicated challenges. Me-thods: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new ex-pression vectors. Results: After designing, these vectors could be applied to produce com-plex human recombinant proteins in the human cancer cell lines as production hosts. Con-clusion: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
BioImpacts; ISSN 2228-5660