TY - GEN T1 - Precision deletion of the entire coding sequence of the mod-5 locus causes increase in pharyngeal pumping frequency AU - Brock, Trisha AU - Pop, Stelian AU - Bradford, Chandler AU - Lawson, Jenn AU - Resch, Lauren AU - Hopkins, Christopher DO - 10.17912/W2NP4D UR - https://www.micropublication.org/journals/biology/w2np4d/ AB - ​The C. elegans mod-5 gene encodes a serotonin transporter. The putative null allele contains a 1,688bp deletion leading to a premature stop codon in the resulting sequence (Ranganathan, 2001); however, unspliced the gene is 12,557bp in length from the start to the stop codon. We used CRISPR/Cas9 technology to create a 12,775bp deletion that eliminates all of the mod-5 coding sequence from the genome (Figure 1A). The mod-5(knu383) animals were measured for pharynx pumping frequency using the NemaMetrix ScreenChip (https://doi.org/10.17912/W2CC7Z; protocol described here http://www.knudra.com/p003). Compared with N2, mod-5(knu383) worms showed a statistically significant increase in pumping frequency (p<0.0001) (Figure 1B). For N2, 18 worms were tested in M9 with no stimulation, and 13 worms were tested for mod-5(knu383). PY - 2017 PB - microPublication Biology ER -